Part:BBa_I735003

Cloning CDS PHO80 in psB3C6

We cloned CDS PHO80 in plasmid psB3C6 containing a RFP. In our project this part is used as an output.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal suffix found in sequence at 1
  Illegal EcoRI site found at 2044
  Illegal XbaI site found at 2278
  Illegal XbaI site found at 2381
  Illegal XbaI site found at 2938
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2044
  Illegal NheI site found at 863
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 2050
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2044
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal suffix found in sequence at 2
  Illegal EcoRI site found at 2044
  Illegal XbaI site found at 2278
  Illegal XbaI site found at 2381
  Illegal XbaI site found at 2938
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2044
  Illegal XbaI site found at 2278
  Illegal XbaI site found at 2381
  Illegal XbaI site found at 2938
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal AgeI site found at 453
  Illegal AgeI site found at 777
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2880